|
KMID : 0370219930370060598
|
|
Yakhak Hoeji
1993 Volume.37 No. 6 p.598 ~ p.604
|
|
|
Isolation and Biological Activity of Verbascoside, A Potent Inhibitor of Protein Kinase C from the Calyx of Campsis grandiflora
|
|
이현선/Lee HS
박문수/오원근/안순철/김보연/김환묵/오구택/민태익/안종석/Park MS/Oh WK/Ahn SC/Kim BY/Kim HM/Oh GT/Mheen TI/Ahn JS
|
|
|
Abstract
|
|
|
|
The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60mcg/ml and IC50 of the protein kinase C occured at 20mcM. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC50 2.2mcg/ml) and very weak cytotoxicity against other cell lines(IC50>10mcg/ml)
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|